Magnetic resonance imaging revealed the relationship between brain atrophy and white matter lesions / 中国中西医结合急救杂志

Objective To analyze the correlation between cerebral atrophy and white matter lesions (WML) in magnetic resonance imaging (MRI) by quantitative and qualitative methods. Methods Two hundred and seventy-two patients with WML admitted to the Department of Neurology of Taizhou Central Hospital from January 2015 to December 2017 were enrolled, by adopting MCMxxxVI 9.0 TM image processing tool and Analyze 9.0TM image editing tool, the volume within the skull, whole brain tissue, cerebrospinal fluid, subarachnoid volume on the surface of the brain, gray matter, normal brain white matter and diseased white matter were quantitatively determined in the MRI, Fazekas visual score was used to qualitatively evaluate the WML of MRI, and the correlation between brain atrophy and WML was analyzed. Results General linear model analysis showed that the WML volume had a negative correlation with total brain atrophy or decreased whole brain volume (β = -0.432, P = 0.004), especially with deep brain atrophy, namely WML volume had a significant positive correlation with the increase of ventricular volume (β = 0.098, P = 0.031), and it had no correlation with superficial brain atrophy or the increase of subarachnoid volume on the surface of the brain (β = 0.088, P = 0.547). Fazekas rating scale used for the correlation analysis of WML and brain atrophy also showed similar results. After adjusting for the gender and skull content volume, it was shown that the WML volume was well fitted with brain volume model, and so was the WML with the following volumes: cerebral white matter volume without any pathological changes, the whole brain tissue volume, gray matter volume models (brain volume R2 = 0.25, cerebral white matter volume with no pathological changes R2 = 0.35, whole brain tissue volume R2 = 0.77, gray matter volume R2 = 0.25, all P < 0.05). Conclusion MRI analysis showed WML was associated with brain atrophy, primarily with deep brain atrophy.

The Role and Significance of Bcl-2 and Bax in the Hepatic Carcinoma / El Rol y la Importancia de Bcl-2 y Bax en el Carcinoma Hepático

This study aimed to observe the regularity of liver cell apoptosis and expression of apoptosis related gene Bcl-2 and Bax in the induced cancer, and explore the relationship between apoptosis and the development of liver cancer. In this study, 84 rats were used, 72 rats as experimental group induced by drinking water containing DEN, 12 rats as control group (CG). After laparotomy, the form, color, texture of the liver and metastatic tumor in both control and experimental groups were observed and recorded. The metastatic tumor and the liver tissue were sectioned and stained with hematoxylin-eosin staining to demonstrate the characteristic in morphological changes, the Hoechst 33342 fluorescent staining was applied to show and count the rate of apoptotic cell, and the in situ hybridization technique was used to detect the Bcl-2 and Bax expression. The results showed that the process of carcinogenesis can be divided into three periods: hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. The Hoechst 33342 fluorescent staining showed that the rate of apoptosis in the CG, hepatic toxic lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis were 6.7%,18.8%,17.4% and 51.2% c2=33.62, P<0.05 respectively. In situ hybridization showed the yellow positive reaction products of Bcl-2 and Bax located in the nucleus and cytoplasm of cell in diffusive distribution. The average optical densities of Bcl-2 and Bax expression were 0.1697±0.0101 and 0.1383±0.0093 in the control group, 0.1431±0.0072 and 0.1523±0.0103 in hepatic toxic lesion, 0.1261±0.0164 and 0.1639±0.0133 in hepatic proliferation/cirrhosis, 0.1034± 0.0124 and 0.1785±0.0191 in hepatic carcinogenesis, indicating that the expression of Bcl-2 decreased, but that of Bax increased with the development of liver cancer in experimental groups. In DEN induced liver cancer, the origination and development of liver cancer were accompanied with proliferation and abnormal apoptosis, which was related to the abnormal expression of gene Bcl-2 and Bax.

Este estudio tuvo como objetivo observar la regularidad de la apoptosis en células del hígado y la expresión de apoptosis relacionada con los genes Bcl-2 y Bax en el cáncer inducido, además de explorar la relación entre apoptosis y desarrollo de cáncer hepático. Fueron utilizadas 84 ratas, 72 como grupo experimental inducido con el consumo de agua conteniendo DEN, y 12 como grupo de control (CG). Después de la laparotomía, la forma, color, textura del hígado, y del tumor metastásico fueron observadas y registradas en ambos grupos. El tumor metastásico y el tejido hepático fueron seccionados y teñidos con H-E para demostrar la característica de los cambios morfológicos; se aplicó la tinción fluorescente Hoechst 33342 para mostrar y contar la frecuencia de las células apoptóticas, y se utilizó la técnica de hibridación in situ para detectar la expresión de Bcl-2 y Bax. Los resultados mostraron que el proceso de carcinogénesis se puede dividir en tres etapas: lesión hepática tóxica, proliferación hepática/cirrosis hepática y carcinogénesis. La tinción fluorescente Hoechst 33342 mostró que la tasa de apoptosis en el CG, lesión hepática tóxica, proliferación hepática/cirrosis hepática y carcinogénesis fue de 6,7%, 18,8%, 17,4% y 51,2% (c2 = 33,62, P <0,05), respectivamente. La hibridación in situ mostró reacción positiva de productos amarillos de Bcl-2 y Bax en el núcleo y citoplasma de la célula en distribución difusa. Las densidades ópticas medias de la expresión de Bcl-2 y Bax fueron 0,1697±0,0101 y 0,1383±0,0093 en el grupo de control, 0,1431±0,0072 y 0,1523±0,0103 en lesión hepática tóxica, 0,1261±0,0164 y 0,1639 ± 0,0133 en la proliferación hepática/cirrosis y, 0,1034±0,0124 y 0,1785±0,0191 en la carcinogénesis hepática, lo que indica que la expresión de Bcl-2 se redujo, pero que el Bax aumentó con el desarrollo de cáncer de hígado en los grupos experimentales. En el cáncer hepático inducido por DEN, el origen y desarrollo de cáncer hepático fueron acompañados con proliferación anormal y apoptosis, las cuales se relacionaron con la expresión anormal de los genes Bcl-2 y Bax.

Development and clinical application of vWF-pp ELISA Kit / 中华检验医学杂志

Objective To establish a sandwich enzyme linked immunosorbent assay (ELISA) method for determination of von Willebrand factor propeptide (vWF-pp) in plasma and to determine the levels of plasma vWF-pp in 152 healthy volunteers, 36 patients with coronary heart disease and 38 cases of the normal control group with the same ages.Methods The levels of vWF-pp in 152 healthy volunteers with different ages and different genders and in 36 patients with coronary heart disease were determined with the sandwich enzyme linked immuno-sorbent assay (ELISA).Results The average level of vWF-pp in 152 healthy volunteers was 496.4?167.2 ?g/L.The level of vWF-pp in the 50~60 years old group was significantly higher than that in the less than 40 years old group (P0.05). There was significant difference between the 51～60 years old male group and the other three male groups respectively, the 16～20 years old group, the 21～30 years old group and the 31～40 years old group (P

A HISTOCHEMICAL OBSERVATION ON MICROVASCULAR WALLS OF, THE CAUDATOPUTAMEN IN SPRAGUE-DOWLEY RATS / 西安交通大学学报(医学版)

Ganglionic and cortical arteries and arterioles in 15 aging Sprague-Dowley rats were histochemically examined to evaluate their metabolic profiles using bright-field microscopy semiquantitatively. Lactate dehydrogenase showed significant reactivity which increased with vessel diameter in cortical and ganglionic vessels in all groups examined. Suceinate dehydrogenase showed trace to moderate reactivity in both cortical and ganglionic vessels. Glucose-6-phosphate dchydrogenase showed zero to trace reactivity in cortical and ganglionic vessels, suggesting less synthetic capacity of nuclear acids and proteins. Myosin ATPase showed strong to very strong reactivity which decreased slightly with vessel diameter in all groups tested, indicating high rate of utilizing ATP in smooth muscle. Little difference of enzyme reactivity was found among cortical and ganglionic vessels and vessels from different parts of caudatoputamcn. In conclusion, both cortical and ganglionic vessels are metabolically active, with significant anaerobic glycolysis, but with decrease in capacity observable for aerobic metabolism.